Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE

Protein kinase expression and activity play important roles in diverse cellular functions through regulation of phosphorylation signaling. The most commonly used tools for detecting the protein kinase are protein kinase-specific antibodies, and phosphorylation site-specific antibodies were used for...

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Udgivet i:MethodsX
Main Authors: Sugiyama, Yasunori, Katayama, Syouichi, Kameshita, Isamu, Morisawa, Keiko, Higuchi, Takuma, Todaka, Hiroshi, Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Koike, Tohru, Taniguchi, Taketoshi, Sakamoto, Shuji
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2015
Fag:
Biochemistry, Genetics and Molecular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4703585/
https://ncbi.nlm.nih.gov/pubmed/26844212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2015.11.007
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