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Purification Strategy for Recombinant Forms of the Human Mitochondrial DNA Helicase
In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification of this enzyme facilitates studies of its structure and mechani...
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| Veröffentlicht in: | Methods Mol Biol |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4703102/ https://ncbi.nlm.nih.gov/pubmed/19513671 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-521-3_8 |
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