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Purification Strategy for Recombinant Forms of the Human Mitochondrial DNA Helicase

In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification of this enzyme facilitates studies of its structure and mechani...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Ziebarth, Tawn D., Kaguni, Laurie S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4703102/
https://ncbi.nlm.nih.gov/pubmed/19513671
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-521-3_8
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