Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei

OBJECTIVES: In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY- α-amylase. METHODS: Escherichia coli BL21 used as a host and protein expression was applied using IPTG. SDS-PAGE assay demonstrated the 100 kDa protei...

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Detalhes bibliográficos
Publicado no:Osong Public Health Res Perspect
Main Authors: Ghasemi, Amir, Ghafourian, Sobhan, Vafaei, Sedighe, Mohebi, Reza, Farzi, Maryam, Taherikalani, Morovat, Sadeghifard, Nourkhoda
Formato: Artigo
Idioma:Inglês
Publicado em: Korea Centers for Disease Control and Prevention 2015
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4700765/
https://ncbi.nlm.nih.gov/pubmed/26835242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.phrp.2015.10.003
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