Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei

OBJECTIVES: In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY- α-amylase. METHODS: Escherichia coli BL21 used as a host and protein expression was applied using IPTG. SDS-PAGE assay demonstrated the 100 kDa protei...

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Vydáno v:Osong Public Health Res Perspect
Hlavní autoři: Ghasemi, Amir, Ghafourian, Sobhan, Vafaei, Sedighe, Mohebi, Reza, Farzi, Maryam, Taherikalani, Morovat, Sadeghifard, Nourkhoda
Médium: Artigo
Jazyk:Inglês
Vydáno: Korea Centers for Disease Control and Prevention 2015
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Original Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4700765/
https://ncbi.nlm.nih.gov/pubmed/26835242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.phrp.2015.10.003
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