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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
BACKGROUND AND OBJECTIVES: Pyrococcus woesei is a hyperthermophilic archaea and produces a heat stable polymerase (Pwo polymerase) that has proofreading activity. MATERIALS AND METHODS: In this study, this microorganism was cultured, its DNA was extracted and the pwo gene polymerase was cloned, expr...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Tehran University of Medical Sciences
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3279813/ https://ncbi.nlm.nih.gov/pubmed/22347593 |
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