Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei

BACKGROUND AND OBJECTIVES: Pyrococcus woesei is a hyperthermophilic archaea and produces a heat stable polymerase (Pwo polymerase) that has proofreading activity. MATERIALS AND METHODS: In this study, this microorganism was cultured, its DNA was extracted and the pwo gene polymerase was cloned, expr...

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Autores principales: Ghasemi, Amir, Salmanian, Ali Hatef, Sadeghifard, Nourkhoda, Salarian, Amir Ahmad, Gholi, Mohammad Khalifeh
Formato: Artigo
Lenguaje:Inglês
Publicado: Tehran University of Medical Sciences 2011
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Original Artical
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3279813/
https://ncbi.nlm.nih.gov/pubmed/22347593
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