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Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms

The lagging annotation of bacterial genomes and the inherent genetic complexity of many phenotypes is hindering the discovery of new drug targets and the development of new antimicrobial agents and vaccines. This unit presents Tn-seq, a method that has made it possible to quantitatively determine fi...

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Pubblicato in:Curr Protoc Microbiol
Autori principali: van Opijnen, Tim, Lazinski, David W., Camilli, Andrew
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4696536/
https://ncbi.nlm.nih.gov/pubmed/25641100
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780471729259.mc01e03s36
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