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Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms

The lagging annotation of bacterial genomes and the inherent genetic complexity of many phenotypes is hindering the discovery of new drug targets and the development of new antimicrobials and vaccines. Here we present the method Tn-seq, with which it has become possible to quantitatively determine f...

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Detalhes bibliográficos
Main Authors: van Opijnen, Tim, Camilli, Andrew
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3877651/
https://ncbi.nlm.nih.gov/pubmed/21053251
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780471729259.mc01e03s19
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