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Definitive Localization of Intracellular Proteins: Novel Approach Using CRISPR-Cas9 Genome Editing, with Glucose 6-Phosphate Dehydrogenase as a Model

Studies to determine subcellular localization and translocation of proteins are important because subcellular localization of proteins affects every aspect of cellular function. Such studies frequently utilize mutagenesis to alter amino acid sequences hypothesized to constitute subcellular localizat...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Spencer, Netanya Y., Yan, Ziying, Cong, Le, Zhang, Yulong, Engelhardt, John F., Stanton, Robert C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4695245/
https://ncbi.nlm.nih.gov/pubmed/26576833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.11.002
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