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Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

During development, hematopoietic cells arise from a specialized subset of endothelial cells, hemogenic endothelium (HE). Modeling HE development in vitro is essential for mechanistic studies of the endothelial-hematopoietic transition and hematopoietic specification. Here, we describe a method for...

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Podrobná bibliografie
Vydáno v:J Vis Exp
Hlavní autoři: Elcheva, Irina, Brok-Volchanskaya, Vera, Slukvin, Igor
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692773/
https://ncbi.nlm.nih.gov/pubmed/26710184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52910
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