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Identification of Kinase-substrate Pairs Using High Throughput Screening

We have developed a screening platform to identify dedicated human protein kinases for phosphorylated substrates which can be used to elucidate novel signal transduction pathways. Our approach features the use of a library of purified GST-tagged human protein kinases and a recombinant protein substr...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Reeks, Courtney, Screaton, Robert A.
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692564/
https://ncbi.nlm.nih.gov/pubmed/26383144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53152
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