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Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

A wide range of methods are currently available for determining the dissociation constant between a protein and interacting small molecules. However, most of these require access to specialist equipment, and often require a degree of expertise to effectively establish reliable experiments and analyz...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Vivoli, Mirella, Novak, Halina R., Littlechild, Jennifer A., Harmer, Nicholas J.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692391/
https://ncbi.nlm.nih.gov/pubmed/25285605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51809
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