Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

A wide range of methods are currently available for determining the dissociation constant between a protein and interacting small molecules. However, most of these require access to specialist equipment, and often require a degree of expertise to effectively establish reliable experiments and analyz...

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Vydáno v:J Vis Exp
Hlavní autoři: Vivoli, Mirella, Novak, Halina R., Littlechild, Jennifer A., Harmer, Nicholas J.
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2014
Témata:
Biophysics
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692391/
https://ncbi.nlm.nih.gov/pubmed/25285605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51809
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