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Rapid Multiplex Creation of Escherchia coli Strains Capable of Interfering with Phage Infection through CRISPR
In Escherichia coli, acquisition of new spacers in the course of CRISPR-Cas adaptation is dramatically stimulated by preexisting partial matches between bacterial CRISPR cassette spacer and a protospacer sequence in DNA of infecting bacteriophage or plasmid transformed. This phenomenon, which we ref...
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| Veröffentlicht in: | Methods Mol Biol |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4690200/ https://ncbi.nlm.nih.gov/pubmed/25981471 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2687-9_9 |
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