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Codon Usage in Signal Sequences Affects Protein Expression and Secretion Using Baculovirus/Insect Cell Expression System

By introducing synonymous mutations into the coding sequences of GP64sp and FibHsp signal peptides, the influences of mRNA secondary structure and codon usage of signal sequences on protein expression and secretion were investigated using baculovirus/insect cell expression system. The results showed...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Wang, Yalan, Mao, Yuanhui, Xu, Xiaodong, Tao, Shiheng, Chen, Hongying
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4689351/
https://ncbi.nlm.nih.gov/pubmed/26697848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0145887
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