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Codon Usage in Signal Sequences Affects Protein Expression and Secretion Using Baculovirus/Insect Cell Expression System

By introducing synonymous mutations into the coding sequences of GP64sp and FibHsp signal peptides, the influences of mRNA secondary structure and codon usage of signal sequences on protein expression and secretion were investigated using baculovirus/insect cell expression system. The results showed...

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Pubblicato in:PLoS One
Autori principali: Wang, Yalan, Mao, Yuanhui, Xu, Xiaodong, Tao, Shiheng, Chen, Hongying
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4689351/
https://ncbi.nlm.nih.gov/pubmed/26697848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0145887
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