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PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing
In CRISPR-Cas9 genome editing, the underlying principles for selecting guide RNA (gRNA) sequences that would ensure for efficient target site modification remain poorly understood. Here we show that target sites harbouring multiple protospacer adjacent motifs (PAMs) are refractory to Cas9-mediated r...
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| Vydáno v: | Nat Commun |
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| Hlavní autoři: | , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Nature Publishing Group
2015
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4686818/ https://ncbi.nlm.nih.gov/pubmed/26644285 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms10124 |
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