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Repurposing CRISPR/Cas9 for in situ functional assays
RNAi combined with next-generation sequencing has proven to be a powerful and cost-effective genetic screening platform in mammalian cells. Still, this technology has its limitations and is incompatible with in situ mutagenesis screens on a genome-wide scale. Using p53 as a proof-of-principle target...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3861673/ https://ncbi.nlm.nih.gov/pubmed/24298059 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.227132.113 |
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