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Development and validation of a high throughput, closed tube method for the determination of haemoglobin alpha gene (HBA1 and HBA2) numbers by gene ratio assay copy enumeration-PCR (GRACE-PCR)

BACKGROUND: Deletions of the α-globin genes are the most common genetic abnormalities in the world. Currently multiplex Gap-PCRs are frequently used to identify specific sets of common deletions. However, these assays require significant post-amplification hands on time and cannot be used to identif...

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Detalhes bibliográficos
Publicado no:BMC Med Genet
Main Authors: Turner, Andrew, Sasse, Jurgen, Varadi, Aniko
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4683937/
https://ncbi.nlm.nih.gov/pubmed/26683685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12881-015-0258-y
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