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Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites

Single-copy rrn strains facilitate genetic ribosomal studies in Escherichia coli. Consecutive markerless deletion of rrn operons resulted in slower growth upon inactivation of the fourth copy, which was reversed by supplying transfer RNA genes encoded in rrn operons in trans. Removal of the sixth, p...

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書目詳細資料
發表在:G3 (Bethesda)
Main Authors: Quan, Selwyn, Skovgaard, Ole, McLaughlin, Robert E., Buurman, Ed T., Squires, Catherine L.
格式: Artigo
語言:Inglês
出版: Genetics Society of America 2015
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4683628/
https://ncbi.nlm.nih.gov/pubmed/26438293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.115.022301
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