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Rapid and efficient construction of markerless deletions in the Escherichia coli genome
We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2504295/ https://ncbi.nlm.nih.gov/pubmed/18567910 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn359 |
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