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Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites
Single-copy rrn strains facilitate genetic ribosomal studies in Escherichia coli. Consecutive markerless deletion of rrn operons resulted in slower growth upon inactivation of the fourth copy, which was reversed by supplying transfer RNA genes encoded in rrn operons in trans. Removal of the sixth, p...
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| Udgivet i: | G3 (Bethesda) |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Genetics Society of America
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4683628/ https://ncbi.nlm.nih.gov/pubmed/26438293 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.115.022301 |
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