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Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites

Single-copy rrn strains facilitate genetic ribosomal studies in Escherichia coli. Consecutive markerless deletion of rrn operons resulted in slower growth upon inactivation of the fourth copy, which was reversed by supplying transfer RNA genes encoded in rrn operons in trans. Removal of the sixth, p...

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Detalhes bibliográficos
Publicado no:G3 (Bethesda)
Main Authors: Quan, Selwyn, Skovgaard, Ole, McLaughlin, Robert E., Buurman, Ed T., Squires, Catherine L.
Formato: Artigo
Idioma:Inglês
Publicado em: Genetics Society of America 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4683628/
https://ncbi.nlm.nih.gov/pubmed/26438293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.115.022301
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