Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Staudinger-Bertozzi ligation

A three-step procedure comprising (i) unnatural-amino-acid mutagenesis with 4-azido-phenylalanine, (ii) Staudinger-Bertozzi ligation with a probe-phosphine derivative, and (iii) in vitro reconstitution of RNA polymerase (RNAP) enables the efficient site-specific incorporation of a fluorescent probe,...

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Publicado en:Methods Mol Biol
Autores principales: Chakraborty, Anirban, Mazumder, Abhishek, Lin, Miaoxin, Hasemeyer, Adam, Xu, Qumiao, Wang, Dongye, Ebright, Yon W., Ebright, Richard H.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2015
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4677679/
https://ncbi.nlm.nih.gov/pubmed/25665560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2392-2_6
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