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Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

BACKGROUND: Next-generation transcriptome sequencing (RNA-Seq) has become the standard practice for studying gene splicing, mutations and changes in gene expression to obtain valuable, accurate biological conclusions. However, obtaining good sequencing coverage and depth to study these is impeded by...

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Bibliografske podrobnosti
izdano v:BMC Res Notes
Main Authors: Heera, Rajandas, Sivachandran, Parimannan, Chinni, Suresh V., Mason, Joanne, Croft, Larry, Ravichandran, Manickam, Su Yin, Lee
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4673735/
https://ncbi.nlm.nih.gov/pubmed/26645211
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-015-1726-3
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