Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

BACKGROUND: Next-generation transcriptome sequencing (RNA-Seq) has become the standard practice for studying gene splicing, mutations and changes in gene expression to obtain valuable, accurate biological conclusions. However, obtaining good sequencing coverage and depth to study these is impeded by...

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Bibliographic Details
Published in:BMC Res Notes
Main Authors: Heera, Rajandas, Sivachandran, Parimannan, Chinni, Suresh V., Mason, Joanne, Croft, Larry, Ravichandran, Manickam, Su Yin, Lee
Format: Artigo
Language:Inglês
Published: BioMed Central 2015
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4673735/
https://ncbi.nlm.nih.gov/pubmed/26645211
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-015-1726-3
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