Efficient extraction of small and large RNAs in bacteria for excellent total RNA sequencing and comprehensive transcriptome analysis

BACKGROUND: Next-generation transcriptome sequencing (RNA-Seq) has become the standard practice for studying gene splicing, mutations and changes in gene expression to obtain valuable, accurate biological conclusions. However, obtaining good sequencing coverage and depth to study these is impeded by...

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Pubblicato in:BMC Res Notes
Autori principali: Heera, Rajandas, Sivachandran, Parimannan, Chinni, Suresh V., Mason, Joanne, Croft, Larry, Ravichandran, Manickam, Su Yin, Lee
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2015
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4673735/
https://ncbi.nlm.nih.gov/pubmed/26645211
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-015-1726-3
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