Recombinant Passenger Proteins Can Be Conveniently Purified by One-Step Affinity Chromatography

Fusion tag is one of the best available tools to date for enhancement of the solubility or improvement of the expression level of recombinant proteins in Escherichia coli. Typically, two consecutive affinity purification steps are often necessitated for the purification of passenger proteins. As a f...

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Bibliographische Detailangaben
Veröffentlicht in:PLoS One
Hauptverfasser: Wang, Hua-zhen, Chu, Zhi-zhan, Chen, Chang-chao, Cao, Ao-cheng, Tong, Xin, Ouyang, Can-bin, Yuan, Qi-hang, Wang, Mi-nan, Wu, Zhong-kun, Wang, Hai-hong, Wang, Sheng-bin
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2015
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4671538/
https://ncbi.nlm.nih.gov/pubmed/26641240
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0143598
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