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Characterization of glyoxalase I purified from pig erythrocytes by affinity chromatography

Glyoxalase I (EC 4.4.1.5) was purified about 10000-fold from pig erythrocytes in a yield of approx. 20%. The purification included affinity chromatography on S-hexylglutathione coupled to Sepharose 4B. The purified enzyme normally contained two catalytically active components which were resolved by...

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Detalhes bibliográficos
Main Authors: Aronsson, Anne-Charlotte, Mannervik, Bengt
Formato: Artigo
Idioma:Inglês
Publicado em: 1977
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1164933/
https://ncbi.nlm.nih.gov/pubmed/921763
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