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Purification of polynucleotide phosphorylase by affinity chromatography and some properties of the purified enzymes

A method is described for the preparation of p-aminophenyl oligo(dT)-Sepharose. This matrix has been used for the purification of polynucleotide phosphorylase from both E.coli and B.stearothermophilus. The effects of temperature and pH on the binding of the different enzymes to the matrix have been...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: Smith, John C., Eaton, Michael A.W.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1974
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343455/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4615305/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/1.12.1763
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