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Purification of polynucleotide phosphorylase by affinity chromatography and some properties of the purified enzymes
A method is described for the preparation of p-aminophenyl oligo(dT)-Sepharose. This matrix has been used for the purification of polynucleotide phosphorylase from both E.coli and B.stearothermophilus. The effects of temperature and pH on the binding of the different enzymes to the matrix have been...
Gorde:
| Egile Nagusiak: | , |
|---|---|
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
1974
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| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343455/ https://ncbi.nlm.nih.gov/pubmed/4615305 |
| Etiketak: |
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