Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.
A rapid method for the identification of known proteins separated by two-dimensional gel electrophoresis is described in which molecular masses of peptide fragments are used to search a protein sequence database. The peptides are generated by in situ reduction, alkylation, and tryptic digestion of p...
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| 出版年: | Proc Natl Acad Sci U S A |
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| 主要な著者: | , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
1993
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46643/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8506346/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.11.5011 |
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