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Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.

A rapid method for the identification of known proteins separated by two-dimensional gel electrophoresis is described in which molecular masses of peptide fragments are used to search a protein sequence database. The peptides are generated by in situ reduction, alkylation, and tryptic digestion of p...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Henzel, W J, Billeci, T M, Stults, J T, Wong, S C, Grimley, C, Watanabe, C
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46643/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8506346/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.11.5011
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