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In vivo polyester immobilized sortase for tagless protein purification
BACKGROUND: Laboratory scale recombinant protein production and purification techniques are often complicated, involving multiple chromatography steps and specialized equipment and reagents. Here it was demonstrated that recombinant proteins can be expressed as covalently immobilized to the surface...
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| Veröffentlicht in: | Microb Cell Fact |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4658790/ https://ncbi.nlm.nih.gov/pubmed/26608345 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0385-3 |
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