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In vivo polyester immobilized sortase for tagless protein purification

BACKGROUND: Laboratory scale recombinant protein production and purification techniques are often complicated, involving multiple chromatography steps and specialized equipment and reagents. Here it was demonstrated that recombinant proteins can be expressed as covalently immobilized to the surface...

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Podrobná bibliografie
Vydáno v:Microb Cell Fact
Hlavní autoři: Hay, Iain D., Du, Jinping, Reyes, Patricia Rubio, Rehm, Bernd H. A.
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4658790/
https://ncbi.nlm.nih.gov/pubmed/26608345
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0385-3
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