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In vivo polyester immobilized sortase for tagless protein purification

BACKGROUND: Laboratory scale recombinant protein production and purification techniques are often complicated, involving multiple chromatography steps and specialized equipment and reagents. Here it was demonstrated that recombinant proteins can be expressed as covalently immobilized to the surface...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Hay, Iain D., Du, Jinping, Reyes, Patricia Rubio, Rehm, Bernd H. A.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4658790/
https://ncbi.nlm.nih.gov/pubmed/26608345
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0385-3
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