Establishment of a simple assay in vitro for hepatitis C virus NS3 serine protease based on recombinant substrate and single-chain protease

AIM: To establish a simple and convenient assay in vitro for the Hepatitis C virus NS3 serine protease based on the recombinant protease and substrate, and to evaluate its feasibility in screening the enzyme inhibitors. METHODS: Based on the crystallographic structure of hepatitis C virus (HCV) seri...

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Bibliographic Details
Published in:World J Gastroenterol
Main Authors: Du, Gui-Xin, Hou, Li-Hua, Guan, Rong-Bin, Tong, Yi-Gang, Wang, Hai-Tao
Format: Artigo
Language:Inglês
Published: Baishideng Publishing Group Inc 2002
Subjects:
Viral Hepatitis
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4656386/
https://ncbi.nlm.nih.gov/pubmed/12439931
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v8.i6.1088
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