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Simple and efficient production of embryonic stem cell-embryo chimeras by coculture.

A method for the production of embryonic stem (ES) cell-embryo chimeras was developed that involves the simple coculture of eight-cell embryos on a lawn of ES cells. After coculture, the embryos with ES cells attached are transferred to normal embryo culture medium and allowed to develop to the blas...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wood, S A, Pascoe, W S, Schmidt, C, Kemler, R, Evans, M J, Allen, N D
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1993
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC46556/
https://ncbi.nlm.nih.gov/pubmed/8506303
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