Simple and efficient production of embryonic stem cell-embryo chimeras by coculture.

A method for the production of embryonic stem (ES) cell-embryo chimeras was developed that involves the simple coculture of eight-cell embryos on a lawn of ES cells. After coculture, the embryos with ES cells attached are transferred to normal embryo culture medium and allowed to develop to the blas...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Wood, S A, Pascoe, W S, Schmidt, C, Kemler, R, Evans, M J, Allen, N D
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1993
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46556/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8506303/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.10.4582
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