Properties of short double-stranded RNAs carrying randomized base pairs: toward better controls for RNAi experiments

Short interfering RNAs (siRNAs) are mediators of RNA interference (RNAi), a commonly used technique for selective down-regulation of target gene expression. Using an equimolar mixture of A, G, C, and U phosphoramidites during solid-phase synthesis, we introduced degenerate positions into RNA guide a...

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Vydáno v:RNA
Hlavní autoři: Zagalak, Julian A., Menzi, Mirjam, Schmich, Fabian, Jahns, Hartmut, Dogar, Afzal M., Wullschleger, Florian, Towbin, Harry, Hall, Jonathan
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2015
Témata:
Method
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4647466/
https://ncbi.nlm.nih.gov/pubmed/26516083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.053637.115
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