“Newton’s cradle” proton relay with amide–imidic acid tautomerization in inverting cellulase visualized by neutron crystallography

Hydrolysis of carbohydrates is a major bioreaction in nature, catalyzed by glycoside hydrolases (GHs). We used neutron diffraction and high-resolution x-ray diffraction analyses to investigate the hydrogen bond network in inverting cellulase PcCel45A, which is an endoglucanase belonging to subfamily...

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Detalhes bibliográficos
Publicado no:Sci Adv
Main Authors: Nakamura, Akihiko, Ishida, Takuya, Kusaka, Katsuhiro, Yamada, Taro, Fushinobu, Shinya, Tanaka, Ichiro, Kaneko, Satoshi, Ohta, Kazunori, Tanaka, Hiroaki, Inaka, Koji, Higuchi, Yoshiki, Niimura, Nobuo, Samejima, Masahiro, Igarashi, Kiyohiko
Formato: Artigo
Idioma:Inglês
Publicado em: American Association for the Advancement of Science 2015
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4643802/
https://ncbi.nlm.nih.gov/pubmed/26601228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.1500263
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