Cryoprotectant-free high-pressure cooling and dynamic nuclear polarization for more sensitive detection of hydrogen in neutron protein crystallography

To improve the sensitivity of hydrogen detection using neutrons, a proton-polarization technique together with a high-pressure cooling method is necessary. The highest pressure (200 MPa) used in the experiment described here enabled relatively large protein crystals to be cooled without any cryoprot...

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Detalles Bibliográficos
Publicado en:Acta Crystallogr D Struct Biol
Main Authors: Tanaka, Ichiro, Komatsuzaki, Naoya, Yue, Wen-Xue, Chatake, Toshiyuki, Kusaka, Katsuhiro, Niimura, Nobuo, Miura, Daisuke, Iwata, Takahiro, Miyachi, Yoshiyuki, Nukazuka, Genki, Matsuda, Hiroki
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2018
Assuntos:
Research Papers
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6079630/
https://ncbi.nlm.nih.gov/pubmed/30082514
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2059798318005028
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