Phase-diagram-guided method for growth of a large crystal of glycoside hydrolase family 45 inverting cellulase suitable for neutron structural analysis

Neutron protein crystallography (NPC) is a powerful tool for determining the hydrogen position and water orientation in proteins, but a much larger protein crystal is needed for NPC than for X-ray crystallography, and thus crystal preparation is a bottleneck. To obtain large protein crystals, it is...

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Hauptverfasser: Nakamura, Akihiko, Ishida, Takuya, Fushinobu, Shinya, Kusaka, Katsuhiro, Tanaka, Ichiro, Inaka, Koji, Higuchi, Yoshiki, Masaki, Mika, Ohta, Kazunori, Kaneko, Satoshi, Niimura, Nobuo, Igarashi, Kiyohiko, Samajima, Masahiro
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2013
Schlagworte:
Diffraction Structural Biology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3795544/
https://ncbi.nlm.nih.gov/pubmed/24121328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0909049513020943
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