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I-SceI-mediated double-strand DNA breaks stimulate efficient gene targeting in the industrial fungus Trichoderma reesei
Targeted integration of expression cassettes for enzyme production in industrial microorganisms is desirable especially when enzyme variants are screened for improved enzymatic properties. However, currently used methods for targeted integration are inefficient and result in low transformation frequ...
Zapisane w:
| Wydane w: | Appl Microbiol Biotechnol |
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| Główni autorzy: | , , , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
Springer Berlin Heidelberg
2015
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4643118/ https://ncbi.nlm.nih.gov/pubmed/26272087 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-015-6829-1 |
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