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I-SceI-mediated double-strand DNA breaks stimulate efficient gene targeting in the industrial fungus Trichoderma reesei

Targeted integration of expression cassettes for enzyme production in industrial microorganisms is desirable especially when enzyme variants are screened for improved enzymatic properties. However, currently used methods for targeted integration are inefficient and result in low transformation frequ...

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Bibliografski detalji
Izdano u:Appl Microbiol Biotechnol
Glavni autori: Ouedraogo, Jean Paul, Arentshorst, Mark, Nikolaev, Igor, Barends, Sharief, Ram, Arthur F. J.
Format: Artigo
Jezik:Inglês
Izdano: Springer Berlin Heidelberg 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4643118/
https://ncbi.nlm.nih.gov/pubmed/26272087
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-015-6829-1
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