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Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9

The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step ‘pop in & out’ targeting approach facilitates this task. See related Research article: http://www.genomebio...

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Publicat a:Genome Biol
Autors principals: Kühn, Ralf, Chu, Van Trung
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4640370/
https://ncbi.nlm.nih.gov/pubmed/26553112
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0810-2
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