Targeted analysis of recombinant NF kappa B (RelA/p65) by denaturing and native top down mass spectrometry

Measuring post-translational modifications on transcription factors by targeted mass spectrometry is hampered by low protein abundance and inefficient isolation. Here, we utilized HaloTag technology to overcome these limitations and evaluate various top down mass spectrometry approaches for measurin...

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Detaylı Bibliyografya
Yayımlandı:J Proteomics
Asıl Yazarlar: Savaryn, John Paul, Skinner, Owen S., Fornelli, Luca, Fellers, Ryan T., Compton, Philip D., Terhune, Scott S., Abecassis, Mike M., Kelleher, Neil L.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2015
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4633404/
https://ncbi.nlm.nih.gov/pubmed/25952688
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jprot.2015.04.025
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