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Targeted analysis of recombinant NF kappa B (RelA/p65) by denaturing and native top down mass spectrometry
Measuring post-translational modifications on transcription factors by targeted mass spectrometry is hampered by low protein abundance and inefficient isolation. Here, we utilized HaloTag technology to overcome these limitations and evaluate various top down mass spectrometry approaches for measurin...
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| Publicado no: | J Proteomics |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4633404/ https://ncbi.nlm.nih.gov/pubmed/25952688 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jprot.2015.04.025 |
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