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Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe

Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through homologous recombination. Despite the advanta...

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Detalhes bibliográficos
Publicado no:Open Biol
Main Authors: Kakui, Yasutaka, Sunaga, Tomonari, Arai, Kunio, Dodgson, James, Ji, Liang, Csikász-Nagy, Attila, Carazo-Salas, Rafael, Sato, Masamitsu
Formato: Artigo
Idioma:Inglês
Publicado em: The Royal Society 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4632507/
https://ncbi.nlm.nih.gov/pubmed/26108218
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1098/rsob.150054
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