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Plasmid Construction Using Recombination Activity in the Fission Yeast Schizosaccharomyces pombe
BACKGROUND: Construction of plasmids is crucial in modern genetic manipulation. As of now, the common method for constructing plasmids is to digest specific DNA sequences with restriction enzymes and to ligate the resulting DNA fragments with DNA ligase. Another potent method to construct plasmids,...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2010
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2836380/ https://ncbi.nlm.nih.gov/pubmed/20300182 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0009652 |
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