High-frequency cotransformation by copolymerization of plasmids in the fission yeast Schizosaccharomyces pombe.
We have developed a high-frequency cotransformation system which is useful in introducing nonreplicating circular DNA plasmids into the fission yeast Schizosaccharomyces pombe. This system depends on two factors: the ability of the ural-complementing helper plasmids pFYM2 and pFYM225 to propagate au...
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| Publicado no: | Mol Cell Biol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis
1984
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368774/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6717440/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.4.4.651-656.1984 |
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